Effects of rheumatoid sera on fibroblast proliferation and hyaluronic acid synthesis.

HE rheumatoid inflammatory process T is characterized by uncontrolled connective tissue cell proliferation, overproduction of specialized cell products, such as hyaluronic acid, colonization of affected tissues by various types of “inflammatory cells,” and alterations in the microvasculature. Once established, this complex process is “self-perpetuating” and leads to destruction of capsular structures, cartilage, and bone with consequent impairment of articular function. To identify and measure the important interrelationships in the rheumatoid granuloma we have employed cell culture techniques which permit intensive study of isolated segments of the problem in a simplified system. There is little literature dealing with the consequences of incorporating rheumatoid sera into nutrient medium to support humean articular cells in uitro. One study concluded that the ability of synovial cells to spread on glass in tissue culture was inhibited by human serum, and that rheurelated to the titer of rheumatoid factor as measured by latex fixation. An improved method for measuring acid mucopolysaccharide in tissue culture medium is described.